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- Title
ESTUDO DA CONSERVAÇÃO DE MATERIAL BIOLÓGICO COM A TECNOLOGIA FTA-Card®.
- Authors
Vinicius Silveira, Cleypson; Benitez, Angelica; Veleh Rivas, Açucena; Fruehwirth, Marcelo; Delai, Robson Michael
- Abstract
Whatman FTA-Card® is a chemically treated filter paper intended for the collection, transport, and storage of samples for later extraction of nucleic acids. FTA-Card® technology is used to keep DNA and RNA stable at room temperature and can be used to fix a wide variety of organic material or tissues. Tests were carried out to certify its efficiency in the conservation of the material to be analyzed in order to eliminate the cold conservation chain, speeding up the process and decreasing the costs of performing molecular tests associated with the diagnosis of pathologies. By using this method, biological material can be sent by postal transport services, reducing costs and making diagnoses from more remote areas feasible. Samples of feline specimens were tested in the form of whole blood and serum, using the Magazorb RNA Total mini-prep kit (Promega®, USA) for the extraction. Diagnosis was performed using real-time PCR technique to amplify the mammalian CI2 gene in order to visualize the effectiveness in conserving nucleic acids.
- Publication
Arquivos de Ciências Veterinárias e Zoologia da Unipar, 2021, Vol 24, Issue 1, p1
- ISSN
1415-8167
- Publication type
Article
- DOI
10.25110/arqvet.v24i1cont.2021.7659