We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
生物菌剂对土壤微生物群落结构和功能的影响.
- Authors
沙月霞; 黄泽阳; 李云翔; 赵沛
- Abstract
This study explored the impact of microbial agents on the structure of the microbial community and functional genes in maize farmland. The metagenomic library was used to analyze the soil microbial community structure, gene abundance of nitrifying functional microorganisms, functional annotation and diversity of carbohydrate-active enzymes (CAZy) and antibiotic resistant genes (ARGs). The results indicated that the abundance of soil microorganisms Proteobacteria, Actinobacteria, and Firmicutes increased after soaking the seed in Wugufengsu and combining it with microbial agents. In addition, the abundance of Acidobacteriotia, Gemmatimonadetes, Chloroflexi, and Candidatus Rokubacteria also decreased. The abundance of Thaumarchaeota and Euryarchaeota in archaea increased by 26.41% and 9.09%, and the abundance of Ascomycota, Basidiomycota and Chytridiomycota in fungi decreased. As for soil microbial genus, the relative abundance of Gemmatimona decreased by 9.76%, the abundance of Bacillus increased by more than 0.77 times, and the abundances of Nitrososphaera, Nitrospira and Candidatus_Nitrosocosmicus increased by more than 20%. Following the application of a microbial agent, the abundance of polysaccharide lyases (PLs) and carbohydrate binding modules (CBMs) increased. Furthermore, the abundance of bacitracin in ARGs genes decreased while the abundance of fluoroquinolone, chloramphenicol, aminoglycoside, beta-lactam increased. In particular, the gene abundance of ammonia oxidizing microorganisms (AOA and AOB) and nitrite oxidizing microorganisms (NOB) increased. The first two main components of PCA explained 98.78% of the class level CAZy difference and 59.33% of the sample level ARGs difference. NMDS and ANOSIM analysis results showed that there were differences in CAZy and ARGs resistance genes across treatments. In conclusion, soaking the seeds in Wugufengsu and combining them with microbial agents has the potential to significantly change the structure of soil microbial community in maize, increase the gene abundance of nitrifying functional microorganisms, optimize the proportion of soil microbial in CAZy, reduce bacitracin pollution in ARGs, and improve the micro-ecological environment of maize plant growth.
- Subjects
NITRIFYING bacteria; SOIL microbiology; SOIL structure; POLYSACCHARIDES; MICROBIAL communities; BACITRACIN; CORN seeds
- Publication
Journal of Agro-Environment Science, 2022, Vol 41, Issue 12, p2752
- ISSN
1672-2043
- Publication type
Article
- DOI
10.11654/jaes.2022-1042