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- Title
The NLR immune receptor ADR1 and lipase-like proteins EDS1 and PAD4 mediate stomatal immunity in Nicotiana benthamiana and Arabidopsis.
- Authors
Wang, Hanling; Song, Susheng; Gao, Shang; Yu, Qiangsheng; Zhang, Haibo; Cui, Xiulin; Fan, Jun; Xin, Xiufang; Liu, Yule; Staskawicz, Brian; Qi, Tiancong
- Abstract
In the presence of pathogenic bacteria, plants close their stomata to prevent pathogen entry. Intracellular nucleotide-binding leucine-rich repeat (NLR) immune receptors recognize pathogenic effectors and activate effector-triggered immune responses. However, the regulatory and molecular mechanisms of stomatal immunity involving NLR immune receptors are unknown. Here, we show that the Nicotiana benthamiana RPW8-NLR central immune receptor ACTIVATED DISEASE RESISTANCE 1 (NbADR1), together with the key immune proteins ENHANCED DISEASE SUSCEPTIBILITY 1 (NbEDS1) and PHYTOALEXIN DEFICIENT 4 (NbPAD4), plays an essential role in bacterial pathogen- and flg22-induced stomatal immunity by regulating the expression of salicylic acid (SA) and abscisic acid (ABA) biosynthesis or response-related genes. NbADR1 recruits NbEDS1 and NbPAD4 in stomata to form a stomatal immune response complex. The transcription factor NbWRKY40e, in association with NbEDS1 and NbPAD4, modulates the expression of SA and ABA biosynthesis or response-related genes to influence stomatal immunity. NbADR1, NbEDS1, and NbPAD4 are required for the pathogen infection-enhanced binding of NbWRKY40e to the ISOCHORISMATE SYNTHASE 1 promoter. Moreover, the ADR1-EDS1-PAD4 module regulates stomatal immunity in Arabidopsis (Arabidopsis thaliana). Collectively, our findings show the pivotal role of the core intracellular immune receptor module ADR1-EDS1-PAD4 in stomatal immunity, which enables plants to limit pathogen entry. Intracellular receptor NbADR1 forms a module with immunity proteins NbEDS1 and NbPAD4, functioning together with transcription factor NbWRKY40e to mediate stomatal immunity in Nicotiana benthamiana.
- Subjects
NICOTIANA benthamiana; STOMATA; IMMUNITY; NATURAL immunity; ARABIDOPSIS; COAT proteins (Viruses)
- Publication
Plant Cell, 2024, Vol 36, Issue 2, p427
- ISSN
1040-4651
- Publication type
Article
- DOI
10.1093/plcell/koad270