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- Title
The conjugative plasmid SLP2 of Streptomyces lividans is a 50 kb linear molecule.
- Authors
Chen, Carton W.; Tin-Wein Yu; Yi-Shing Lin; Kieser, Helen M.; Hopwood, David A.
- Abstract
The SLP2 plasmid had previously been demonstrated genetically to exist in <em>Streptomyces lividans</em> by its ability to promote conjugation and to elicit 'pocks' on recipient (SLP2-) cultures, but it had not been physically detected. Using pulsed-field gel electrophoresis, a 50 kb linear DNA was isolated from SLP2+ but not SLP2- strains of <em>S. lividans</em>, and from <em>Streptomyces coelicolor</em> and <em>Streptomyces parvulus</em> strains to which SLP2 had been transferred by conjugation or transformation. We conclude that this linear DNA is SLP2. The terminal fragments of SLP2 were cloned. The determined sequences revealed a 44 bp imperfect terminal inverted repeat. The terminal 12 bp sequence of SLP2 was identical to those of two other <em>Streptomyces</em> linear plasmids, pSLA2 and pSCL, and similar to the terminal sequences of another <em>Streptomyces</em> linear plasmid, SCP1. The termini of SLP2 DNA were resistant to digestion by λ exonuclease and Exolll. A truncated (probably crippled) copy of Tn<em>4811</em> is present on the plasmid. While the SLP2 plasmid exists as a free form in the host, a 15.7 kb sequence corresponding to the segment of SLP2 from Tn<em>4811</em> to the right terminus is also present (at a copy number similar to the free form) elsewhere in the genome of <em>S. lividans</em>. Furthermore, SLP2 is partially homologous to a newly discovered 650 kb linear plasmid in <em>S. parvulus</em>.
- Subjects
STREPTOMYCETACEAE; MOBILE genetic elements; DNA; GENES; BACTERIAL conjugation; COLLOIDS; CYTOPLASMIC inheritance; GENETICS; GENOMES
- Publication
Molecular Microbiology, 1993, Vol 7, Issue 6, p925
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.1993.tb01183.x