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- Title
Complete genome sequence of <italic>Bacillus velezensis</italic> 157 isolated from <italic>Eucommia ulmoides</italic> with pathogenic bacteria inhibiting and lignocellulolytic enzymes production by SSF.
- Authors
Chen, Long; Gu, Wei; Xu, Hai-yan; Yang, Gui-Lian; Shan, Xiao-Feng; Chen, Guang; Wang, Chun-Feng; Qian, Ai-Dong
- Abstract
<italic>Bacillus velezensis</italic> 157 was isolated from the bark of <italic>Eucommia ulmoides</italic>, and exhibited antagonistic activity against a broad spectrum of pathogenic bacteria and fungi. Moreover, <italic>B. velezensis</italic> 157 also showed various lignocellulolytic activities including cellulase, xylanase, α-amylase, and pectinase, which had the ability of using the agro-industrial waste (soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw) under solid-state fermentation and obtained several industrially valuable enzymes. Soybean meal appeared to be the most efficient substrate for the single fermentation of <italic>B. velezensis</italic> 157. Highest yield of pectinase (19.15 ± 2.66 U g−1), cellulase (46.69 ± 1.19 U g−1) and amylase (2097.18 ± 15.28 U g−1) was achieved on untreated soybean meal. Highest yield of xylanase (22.35 ± 2.24 U g−1) was obtained on untreated wheat bran. Here, we report the complete genome sequence of the <italic>B. velezensis</italic> 157, composed of a circular 4,013,317 bp chromosome with 3789 coding genes and a G + C content of 46.41%, one circular 8439 bp plasmid and a G + C content of 40.32%. The genome contained a total of 8 candidate gene clusters (bacillaene, difficidin, macrolactin, butirosin, bacillibactin, bacilysin, fengycin and surfactin), and dedicates over 15.8% of the whole genome to synthesize secondary metabolite biosynthesis. In addition, the genes encoding enzymes involved in degradation of cellulose, xylan, lignin, starch, mannan, galactoside and arabinan were found in the <italic>B. velezensis</italic> 157 genome. Thus, the study of <italic>B. velezensis</italic> 157 broadened that <italic>B. velezensis</italic> can not only be used as biocontrol agents, but also has potentially a wide range of applications in lignocellulosic biomass conversion.
- Subjects
NUCLEOTIDE sequencing; BACILLUS (Bacteria); EUCOMMIA ulmoides; PATHOGENIC bacteria; LIGNOCELLULOSE; PECTIC enzymes
- Publication
3 Biotech, 2018, Vol 8, Issue 2, p0
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1125-2