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- Title
Even after UVA-exposure will nitric oxide protect cells from reactive oxygen intermediate-mediated apoptosis and necrosis.
- Authors
Suschek, C V; Briviba, K; Bruch-Gerharz, D; Sies, H; Kröncke, K D; Kolb-Bachofen, V
- Abstract
Reactive oxygen species (ROS) play a pivotal role in UVAinduced cell damage. As expression of the inducible nitric oxide synthase (iNOS) is a normal response of human skin to UV radiation we examined the role of nitric oxide (NO) as a protective agent during or even after UVA[sub 1]- or ROS-exposure against apoptosis or necrosis of rat endothelial cells. When added during or up to 2 h subsequent to UVA[sub 1] or ROS exposure the NO-donor S-nitroso-cysteine (SNOC) at concentrations from 100-1000 µM significantly protects from both apoptosis as well as necrosis. The NO-mediated protection strongly correlates with complete inhibition of lipid peroxidation (sixfold increase of malonedialdehyde formation in untreated versus 1.2-fold with 1 mM SNOC). NO-mediated protection of membrane function was also shown by the inhibition of cytochrome c leakage in UVA[sub 1] treated cells, a process not accompanied by alterations in Bax and Bcl-2 protein levels. Thus, the experiments presented demonstrate that NO exposure during or even after a ROSmediated toxic insult fully protects from apoptosis or necrosis by maintaining membrane integrity and function.
- Subjects
REACTIVE oxygen species; NITRIC oxide; APOPTOSIS; NECROSIS
- Publication
Cell Death & Differentiation, 2001, Vol 8, Issue 5, p515
- ISSN
1350-9047
- Publication type
Article
- DOI
10.1038/sj.cdd.4400839