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- Title
Surface characterization, hemo- and cytocompatibility of segmented poly(dimethylsiloxane)-based polyurethanes.
- Authors
Pergal, Marija V.; Vasiljević-Radović, Dana; Nestorov, Jelena; Tovilović-Kovačević, Gordana; Jovančić, Petar; Djonlagić, Jasna; Pezo, Lato
- Abstract
Segmented polyurethanes based on poly(dimethylsiloxane), currently used for biomedical applications, have sub-optimal biocompatibility which reduces their efficacy. Improving the endothelial cell attachment and blood-contacting properties of PDMS-based copolymers would substantially improve their clinical applications. We have studied the surface properties and in vitro biocompatibility of two series of segmented poly(urethane-dimethylsiloxane) s (SPU-PDMS) based on hydroxypropyl- and hydroxyethoxypropyl-terminated PDMS with potential applications in blood-contacting medical devices. SPU-PDMS copolymers were characterized by contact angle measurements, surface free energy determination (calculated using the van Oss-Chaudhury-Good and Owens-Wendt methods), and atomic force microscopy. The biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact assay, before and after pre-treatment of copolymers with multicomponent protein mixture, as well as by a competitive blood-protein adsorption assay. The obtained results suggested good blood compatibility of synthesized copolymers. All copolymers exhibited good resistance to fibrinogen adsorption and all favored albumin adsorption. Copolymers based on hydroxyethoxypropyl-PDMS had lower hydrophobicity, higher surface free energy and better microphase separation in comparison with hydroxypropyl-PDMS-based copolymers, which promoted better endothelial cell attachment and growth on the surface of these polymers as compared to hydroxypropyl- PDMS-based copolymers. The results showed that SPU-PDMS copolymers display good surface properties, depending on the type of soft PDMS segments, which can be tailored for biomedical application requirements such as biomedical devices for short- and long-term uses.
- Subjects
POLYURETHANES; SURFACE analysis; DIMETHYLPOLYSILOXANES; BIOCOMPATIBILITY; CELL adhesion
- Publication
Chemical Industry / Hemijska Industrija, 2014, Vol 68, Issue 6, p731
- ISSN
0367-598X
- Publication type
Article
- DOI
10.2298/HEMIND141103082P