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- Title
Chitosan Nanoparticles of Gamma-Oryzanol: Formulation, Optimization, and <italic>In vivo</italic> Evaluation of Anti-hyperlipidemic Activity.
- Authors
Rawal, Tejal; Mishra, Neha; Jha, Abhishek; Bhatt, Apurva; Tyagi, Rajeev K.; Panchal, Shital; Butani, Shital
- Abstract
The elevated blood levels of cholesterol and low-density lipoproteins result in hyperlipidemia. The available expensive prophylactic treatments are kindred with severe side effects. Therefore, we fabricated the polymeric nanoparticles of gamma-oryzanol to achieving the improved efficacy of drug. The nanoparticles were prepared by ionic gelation method and optimized using 23 full factorial design taking drug/polymer ratio (<italic>X</italic>1), polymer/cross linking agent ratio (<italic>X</italic>2), and stirring speed (<italic>X</italic>3) as independent variables. The average particle size, percentage entrapment efficiency, and <italic>in vitro</italic> drug release at 2, 12, and 24 h were selected as response parameters. The factorial batches were statistically analyzed and optimized. The optimized nanoparticles were characterized with respect to particle size (141 nm) and zeta potential (+ 6.45 mV). Results obtained with the prepared and characterized formulation showed 83% mucoadhesion towards the intestinal mucosa. The <italic>in vitro</italic> findings were complemented well by <italic>in vivo</italic> anti-hyperlipidemic activity of developed formulation carried out in Swiss albino mouse model. The <italic>in vivo</italic> studies showed improved atherogenic index, malondialdehyde, and superoxide dismutase levels in poloxamer-407-induced hyperlipidemic animals when treated with oryzanol and gamma-oryzanol nanoformulation. Based on our findings, we believe that chitosan-mediated delivery of gamma-oryzanol nanoparticles might prove better in terms of anti-hyperlipidemic therapeutics.
- Publication
AAPS PharmSciTech, 2018, Vol 19, Issue 4, p1894
- ISSN
1530-9932
- Publication type
Article
- DOI
10.1208/s12249-018-1001-8