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- Title
Increased expression of endothelial lipase in rat models of hypertension
- Authors
Shimokawa, Yasushi; Hirata, Ken-ichi; Ishida, Tatsuro; Kojima, Yoko; Inoue, Nobutaka; Quertermous, Thomas; Yokoyama, Mitsuhiro
- Abstract
Abstract: Objective: To gain a better understanding of the involvement of endothelial lipase (EL) in vascular disease, we examined whether the EL expression is regulated in animal models of hypertension. Methods: The rat cDNA homologue of EL was identified using reverse transcription-polymerase chain reaction. Cultured rat aortic smooth muscle cells were stimulated with angiotensin II (Ang II) and phorbol 12-myristate 13-acetate (PMA), and EL mRNA expression was analyzed by Northern blotting. EL mRNA levels in tissues from stroke-prone spontaneously hypertensive rats (SHR-SP) and Ang II-induced hypertensive rats were evaluated using RNase protection assays. Results: Rat EL cDNA encoded a protein containing 493 amino acid residues including a signal peptide, and shares 91.9% and 80.9% sequence homology with murine and human EL, respectively. Northern blotting revealed that EL was expressed in a wide range of rat tissues. In cultured rat aortic smooth muscle cells, Ang II and PMA increased EL mRNA levels by 2.9- and 3.3-fold, respectively. In Ang II-induced hypertensive rats, EL expression was upregulated in the aorta, heart, and lung. In SHR-SP, EL expression was upregulated in the aorta and heart. Conclusion: EL expression is increased in rat models of hypertension. Thus, EL might have a role in the local pathophysiology of vascular diseases.
- Subjects
LIPASES; HYPERTENSION; VASCULAR diseases; SMOOTH muscle
- Publication
Cardiovascular Research, 2005, Vol 66, Issue 3, p594
- ISSN
0008-6363
- Publication type
Article
- DOI
10.1016/j.cardiores.2005.01.013