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- Title
Global Ca<sup>2+</sup> Signaling Drives Ribbon-Independent Synaptic Transmission at Rod Bipolar Cell Synapses.
- Authors
Mehta, Bhupesh; Jiang-Bin Ke; Lei Zhang; Baden, Alexander D.; Markowitz, Alexander L.; Nayak, Subhashree; Briggman, Kevin L.; Zenisek, David; Singer, Joshua H.
- Abstract
Ribbon-type presynaptic active zones are a hallmark of excitatory retinal synapses, and the ribbon organelle is thought to serve as the organizing point of the presynaptic active zone. Imaging of exocytosis from isolated retinal neurons, however, has revealed ectopic release (i.e., release away from ribbons) in significant quantities. Here, we demonstrate in an in vitro mouse retinal slice preparation that ribbon-independent release from rod bipolar cells activates postsynaptic AMPARs on AII amacrine cells. This form of release appears to draw on a unique, ribbon-independent, vesicle pool. Experimental, anatomical, and computational analyses indicate that it is elicited by a significant, global elevation of intraterminal [Ca2+] arising following local buffer saturation. Our observations support the conclusion that ribbon-independent release provides a read-out of the average behavior of all of the active zones in a rod bipolar cell's terminal.
- Subjects
NEURAL transmission; EXOCYTOSIS; SYNAPTIC vesicles; CALCIUM ions; RIBBONS; RETINAL rod photoreceptor cells; PRESYNAPTIC receptors; CELLULAR signal transduction
- Publication
Journal of Neuroscience, 2014, Vol 34, Issue 18, p6233
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.5324-13.2014