We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of the microbial diverisity and the mechanism of simultaneous nitrification and denitrification in high nitrogen environments.
- Authors
Zhang, X. Y.; Zeng, Y. W.; Tao, R. D.; Zhang, M.; Zheng, M. M.; Qu, M. J.; Mei, Y. J.
- Abstract
Simultaneous nitrification and denitrification (SND) is cost-effective, and therefore it is a promising process for biological nitrogen removal. In this study, SND microbes were obtained through domestication of the sludge from Wuhan municipal wastewater treatment plant to enrich effective nitrogen removal SND microbes, to reveal the microbial structure of SND and the denitrogenation mechanism through the high-throughput sequencing technology and quantitative PCR. When NH4+-N (~ 90.7 mg/) as a sole nitrogen source, the removal efficiency (RE) of NH4+-N in 24 h was 99.35%, and there was no accumulation of NO3−-N or NO2−-N. When NO3−-N (~ 101.79 mg/L) or NO2−-N (~ 106.00 mg/L) was the sole nitrogen source, respectively, it was almost completely removed. Furthermore, when NH4+-N (~ 105.68 mg/L) and NO3−-N (~ 100.40 mg/L) were the composite nitrogen sources, the RE of NH4+-N and NO3−-N were 99.17% and 94.07% in 24 h, respectively. The RE of NH4+-N was up to 99.31% when NH4+-N (~ 102.33 mg/L) and NO2−-N (~ 114.56 mg/L) used as composite nitrogen sources, and NO2−-N was completely removed. Community composition analysis revealed that the dominant phyla were Proteobacteria and Bacteroidetes; and the dominant genera were Paracoccus, Pannonibacter, Taibaiella, Pelagibacterium and Stappia. Gene functional analysis for the nitrogen cycle showed the presence of amoB, hao, narG, nirS, nirK, and nosZ genes; however, nxrA, hzo, hzs, napA, and nasA were not detected. These findings revealed that a possible novel mechanism of nitrogen removal using hao gene to oxidize NH2OH to NO instead of NO2−-N by SND microbes.
- Subjects
WUHAN (China); NITRIFICATION; SEWAGE disposal plants; DENITRIFICATION; NITROGEN cycle; NITROGEN; NITROGEN analysis
- Publication
International Journal of Environmental Science & Technology (IJEST), 2024, Vol 21, Issue 10, p7177
- ISSN
1735-1472
- Publication type
Article
- DOI
10.1007/s13762-024-05463-5