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- Title
Topically Applied Nitric Oxide Induces T-Lymphocyte Infiltration in Human Skin, but Minimal Inflammation.
- Authors
Mowbray, Megan; Xuejing Tan; Wheatley, Paul S.; Morris, Russell E.; Weller, Richard B.
- Abstract
Nitric oxide (NO) plays an important role in the cutaneous response to UV radiation and in cutaneous inflammation. The presence of inducible NO synthase protein in a number of inflammatory dermatoses, coupled with the induction of an intense cutaneous inflammatory infiltrate following topical application of the NO donor-acidified nitrite (NO2−), has set the paradigm of NO being an inflammatory mediator in human skin. Using zeolite NO (Ze–NO), a chemically inert, pure NO donor, we have shown that NO per se produces little inflammation. Biologically, relevant doses of Ze–NO induce a dermal CD4-positive T-cell infiltrate and IFN-γ secretion. In contrast acidified nitrite, releasing equal quantities of NO (measured by dermal microdialysis and cutaneous erythema), induces an intense epidermal infiltrate of macrophages with a similar dermal infiltrate of CD3-, CD4-, CD8-, and CD68-positive cells and neutrophils. Suction blisters were created in Ze–NO-treated and control skin. IFN-γ, but not IL-4, was detected in Ze–NO-treated skin (mean control 0.1±0.07 pg mg−1 protein, mean IFN-γ 0.6±0.4 pg mg−1 protein). We suggest that the potent inflammation induced by acidified NO2− is secondary to the release of additional mediators.Journal of Investigative Dermatology (2008) 128, 352–360; doi:10.1038/sj.jid.5701096; published online 4 October 2007
- Subjects
NITRIC oxide; SKIN diseases; LYMPHOCYTES; ULTRAVIOLET radiation; INFLAMMATORY mediators; BIOLOGICAL transport
- Publication
Journal of Investigative Dermatology, 2008, Vol 128, Issue 2, p352
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/sj.jid.5701096