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- Title
MicroRNA-103 promotes nasopharyngeal carcinoma through targeting TIMP-3 and the Wnt/β-catenin pathway.
- Authors
Zhao, Yigang; Gu, Xiao; Wang, Yanpeng
- Abstract
<bold>Objectives/hypothesis: </bold>To verify how microRNA-103 (miR-103) is involved in nasopharyngeal carcinoma (NPC) development.<bold>Study Design: </bold>Research on the relationship between miR-103 and NPC.<bold>Methods: </bold>Tissue inhibitor of metalloproteinases-3 (TIMP-3) was identified as the theoretical target gene of miR-103, and its regulatory mechanism in NPC was explored by quantitative reverse transcription polymerase chain reaction, Western blot, MTT, transwell, and luciferase reporter assays.<bold>Results: </bold>MiR-103 was upregulated whereas TIMP-3 was markedly decreased in NPC tissue samples. Ectopic expression of miR-103 promoted NPC cell viability, migration, and invasion. In vitro assay showed that TIMP-3 recovered miR-103-mediated promotion of NPC cell viability, migration, and invasion. The expression of Wnt/β-catenin pathway markers (β-catenin and cyclin D1) were enhanced after miR-103 overexpression.<bold>Conclusions: </bold>MiR-103 might play a key role in NPC carcinogenesis by targeting TIMP-3 and affecting the Wnt/β-catenin pathway.<bold>Level Of Evidence: </bold>NA Laryngoscope, 130:E75-E82, 2020.
- Subjects
REVERSE transcriptase polymerase chain reaction; CARCINOMA; PROTEIN metabolism; RNA physiology; CARCINOGENESIS; CELLULAR signal transduction; NASOPHARYNX tumors
- Publication
Laryngoscope, 2020, Vol 130, Issue 3, pE75
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.28045