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- Title
Uncultivated stromal vascular fraction is equivalent to adipose-derived stem and stromal cells on porous polyurethrane scaffolds forming adipose tissue in vivo.
- Authors
Griessl, Michael; Buchberger, Anna‐Maria; Regn, Sybille; Kreutzer, Kilian; Storck, Katharina; Buchberger, Anna-Maria
- Abstract
<bold>Objectives/hypothesis: </bold>To find an alternative approach to contemporary techniques in tissue augmentation and reconstruction, tissue engineering strategies aim to involve adipose-derived stem and stromal cells (ASCs) harboring a strong differentiation potential into various tissue types such as bone, cartilage, and fat.<bold>Study Design: </bold>Animal research.<bold>Methods: </bold>The stromal vascular fraction (SVF) was used directly as a cell source to provide a potential alternative to contemporary ASC-based adipose tissue engineering. Seeded in TissuCol fibrin, we applied ASCs or SVF cells to porous, degradable polyurethane (PU) scaffolds.<bold>Results: </bold>We successfully demonstrated the in vivo generation of volume-stable, well-vascularized PU-based constructs containing host-derived mature fat pads. Seeded human stem cells served as modulators of host-cell migration rather than differentiating themselves. We further demonstrated that preliminary culture of SVF cells was not necessary.<bold>Conclusions: </bold>Our results bring adipose tissue engineering, together with automated processing devices, closer to clinical applicability. The time-consuming and cost-intensive culture and induction of the ASCs is not necessary.<bold>Level Of Evidence: </bold>NA. Laryngoscope, 128:E206-E213, 2018.
- Subjects
ADIPOSE tissues; STROMAL cells; TISSUE engineering; CARTILAGE diseases; STEM cell treatment; BIOLOGICAL models; FIBRIN tissue adhesive; IN vivo studies; RESEARCH methodology; ANIMAL experimentation; TISSUE culture; BIOMEDICAL materials; CELL motility; FAT cells; CONNECTIVE tissue cells; POLYURETHANES
- Publication
Laryngoscope, 2018, Vol 128, Issue 6, pE206
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.27124