We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of scutellarin on contractile behavior of isolated thoracic aorta rings of the mouse.
- Authors
Weimin Yang; Bofferding, April; Lust, Robert M.; Wingard, Christopher J.
- Abstract
The vascular reactivity of scutellarin (SCU), a flavonoid isolated from a plant used in Chinese traditional herbal medicine, was investigated. Thoracic aortas from male ICR mice were isolated and isometric tension measurements were made. In phenylephrine (PE, 1 µM) pre-contracted intact aortic rings, SCU caused dose-dependent relaxations, with an EC50 value of 1200.8 ± 126.6 µM (n = 8). In KCl (30 mM) and U46619 (10 nM) pre-contracted aortas, SCU showed less relaxation. The SCU relaxation in aortas was sharply reduced by endothelium removal, suggesting that it is predominantly endothelium dependent. The vasorelaxation was also significantly inhibited by nitric oxide synthase inhibitor L-NAME (300 µM) and the guanylate cyclase (sGC) inhibitor ODQ (100 µM), but not by an adenylate cyclase (AC) blocker SQ 22536 (100 µM). Moreover, PE dose response curves were significantly suppressed by preincubation with 300 µM and 1000 µM SCU in both endothelium-intact and denuded aortas. The relaxation of SCU were unaffected by the cyclooxygenase inhibitor (1 µM, Indomethacin), adenosine receptor A1 (100 nM, DPCPX), A3 (100nM, MRS-1754) or A2a (100nM, SCH-58261) blockers or thromboxane receptor antagonist (1 µM, SQ-29548). Therefore, 'we concluded that SCU caused vasorelaxation that was endothelium-dependent and involved the NO/sGC/cGMP pathway and inhibited force generated by PE in isolated aortas.
- Subjects
FLAVONOIDS; THORACIC arteries; NITRIC-oxide synthases; GUANYLATE cyclase; ADENYLATE cyclase; THROMBOXANES; AORTA; LABORATORY mice
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1160
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.6.a1160-b