We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Transplantation of cultured rhesus monkey vascular endothelial cells to allogeneic cornea concomitant with stripping of Descemet's membrane.
- Authors
Qin Zhu; Min Wu; Xiaomei Sun; Wenjia Zhang; Zhulin Hu; Hai Liu; Zhu, Qin; Wu, Min; Sun, Xiaomei; Zhang, Wenjia; Hu, Zhulin; Liu, Hai
- Abstract
<bold>Context: </bold>In cases of damaged corneal endothelium cells (CECs) of the eye, transplantation of cultured vascular endothelial cells (VECs) may be a viable method to restore transparency.<bold>Aims: </bold>To evaluate the viability of replacing damaged primate CECs with cultured allogeneic VECs.<bold>Subjects and Methods: </bold>Rhesus monkey VECs (RMVECs) were cultured and proliferating cells were labeled with bromodeoxyuridine (BrdU) in vitro. RMs of the experimental group (n = 6) underwent manual Descemettt membrane stripping with transplantation of RMVECs labeled with BrdU; those in the control group received manual Descemetnt membrane stripping without transplantation. Postoperative evaluations included the transparency and appearance of the corneal graft; distribution and ultrastructural changes of RMVECs on the inner surface of the cornea using scanning and transmission electron microscopy, and immunohistological identification of BrdU.<bold>Results: </bold>At 90 days postsurgery, the corneal grafts of the monkeys in the experimental group retained better transparency than those of the controls, without corneal neovascularization or bullous keratopathy. A layer of cells with positive BrdU staining was found on the posterior surface of the treated corneas in the experimental group, while there was no VEC structure in corneal grafts from the monkeys of the control group.<bold>Conclusions: </bold>RMVECs can grow on the posterior surface of the cornea without Descemet's membrane. Cultured and transplanted RMVECs appeared similar in ultrastructure. VECs can provide a barrier to maintain corneal dehydration and transparency to some extent.
- Subjects
CORNEA surgery; CORNEAL transplantation; RHESUS monkeys; VASCULAR endothelial cells; HOMOGRAFTS; CELL culture; ENDOTHELIUM; ANIMAL experimentation; BIOLOGICAL models; COMPARATIVE studies; CORNEA; CORNEA diseases; EPITHELIAL cells; RESEARCH methodology; MEDICAL cooperation; PRIMATES; RESEARCH; EVALUATION research; DIAGNOSIS; TRANSPLANTATION of organs, tissues, etc.
- Publication
Indian Journal of Ophthalmology, 2015, Vol 63, Issue 8, p665
- ISSN
0301-4738
- Publication type
journal article
- DOI
10.4103/0301-4738.169788