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- Title
Mn<sup>2+</sup>–DNA interactions in aqueous systems: A Raman spectroscopic study.
- Authors
Muntean, Cristina M.; Misselwitz, Rolf; Dostál, Lubomir; Welfle, Heinz
- Abstract
Interaction of natural calf thymus DNA with Mn2+ ions was studied by means of Raman spectroscopy. Spectra of DNA in 10 mM Na-cacodylate buffer, pH 6.2, 10 mM NaCl and in buffer containing Mn2+ ions were measured at room temperature. Mn2+ concentrations varied between 0 and 0.6 M. DNA backbone conformational changes and DNA denaturation were not observed in the concentration range 0 and 0.5 M, however, DNA condensation was observed at a critical concentration of 100 mM Mn2+ that prevented the measurement of Raman spectra. Binding of Mn2+ to the charged phosphate groups of DNA is indicated in the spectra. A high affinity of Mn2+ for guanine N7 was obvious, and binding to adenine was barely suggested.
- Subjects
RAMAN spectroscopy; SPECTRUM analysis; DNA; NUCLEIC acids; MOLECULAR spectra; GENES
- Publication
Spectroscopy: An International Journal, 2006, Vol 20, Issue 1, p29
- ISSN
0712-4813
- Publication type
Article
- DOI
10.1155/2006/429147