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- Title
Predicted expansion of the claudin multigene family
- Authors
Mineta, Katsuhiko; Yamamoto, Yasuko; Yamazaki, Yuji; Tanaka, Hiroo; Tada, Yukiyo; Saito, Kuniaki; Tamura, Atsushi; Igarashi, Michihiro; Endo, Toshinori; Takeuchi, Kosei; Tsukita, Sachiko
- Abstract
Abstract: Claudins (Cldn) are essential membrane proteins of tight junctions (TJs), which form the paracellular permselective barrier. They are produced by a multi-gene family of 24 reported members in mouse and human. Based on a comprehensive search combined with phylogenetic analyses, we identified three novel claudins (claudin-25, -26, and -27). Quantitative RT-PCR revealed that the three novel claudins were expressed in a tissue- and/or developmental stage-dependent manner. Claudins-25 and -26, but not claudin-27, were immunofluorescently localized to TJs when exogenously expressed in cultured MDCK and Eph epithelial cell lines. These findings expand the claudin family to include at least 27 members. Structured summary: Claudin-25 and ZO-1 colocalize by fluorescence microscopy (View interaction) ZO-1 and Claudin-26 colocalize by fluorescence microscopy (View interaction)
- Subjects
PREDICTION models; MEMBRANE proteins; CELL junctions; LABORATORY mice; PHYLOGENY; FLUORESCENCE microscopy; PROTEIN-protein interactions; EPITHELIAL cells; CELL adhesion
- Publication
FEBS Letters, 2011, Vol 585, Issue 4, p606
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.01.028