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- Title
Radioprotection by short-term oxidative preconditioning: Role of manganese superoxide dismutase
- Authors
Belikova, Natalia A.; Glumac, Ashley; Rafikov, Ruslan; Jiang, Jianfei; Greenberger, Joel S.; Kagan, Valerian E.; Bayir, Hülya
- Abstract
Abstract: Manganese superoxide dismutase (MnSOD) is vital to the protection of mitochondria and cells against oxidative stress. Earlier, we demonstrated that catalytically active homo-tetramer of MnSOD can be stabilized by oxidative cross-linking. Here we report that this effect may be translated into increased radioresistance of mouse embryonic cells (MECs) by pre-exposure to oxidative stress. Pre-treatment of MECs with antimycin A, rotenone or H2O2 increased their survival after irradiation. Using MnSOD siRNA, we show that MECs with decreased MnSOD levels displayed a lowered ability to preconditioning. Thus oxidative preconditioning may be used for targeted regulation of MnSOD. Structured summary: MINT-7288408: MnSOD (uniprotkb:P04179) and MnSOD (uniprotkb:P04179) physically interact (MI:0915) by zymography (MI:0512)
- Subjects
HYDROGEN peroxide; IRRADIATION; APOPTOSIS; IONIZING radiation; SUPEROXIDE dismutase; PHOSPHATIDYLSERINES; MITOCHONDRIA; ROTENONE
- Publication
FEBS Letters, 2009, Vol 583, Issue 21, p3437
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.10.013