We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Pectenotoxin-2 represses telomerase activity in human leukemia cells through suppression of hTERT gene expression and Akt-dependent hTERT phosphorylation
- Authors
Kim, Mun-Ock; Moon, Dong-Oh; Kang, Sang-Hyuck; Heo, Moon-Soo; Choi, Yung Hyun; Jung, Jee Hyung; Lee, Jae-Dong; Kim, Gi-Young
- Abstract
Abstract: In this study, we found that pectenotoxin-2 (PTX-2) decreased cell viability and inhibited telomerase activity with downregulation of hTERT expression in human leukemia cells. PTX-2 treatment also reduced c-Myc and Sp1 gene expression and DNA binding activity. Further chromatin immunoprecipitation assay demonstrated that PTX-2 attenuated the binding of c-Myc and Sp1 to the regulatory regions of hTERT. We also observed that PTX-2 treatment attenuated the phosphorylation of Akt, thereby reducing the phosphorylation and nuclear translocation of hTERT. We concluded that PTX-2 suppressed telomerase activity through the transcriptional and post-translational suppression of hTERT and this process precedes cellular differentiation of human leukemia cells. Structured summary: MINT-6742762: hTERT (uniprotkb:O14746) physically interacts (MI:0218) with AKT (uniprotkb:P31749) by anti bait coimmunoprecipitation (MI:0006)
- Subjects
GENE expression; TUMOR suppressor genes; CANCER cells; PHOSPHORYLATION
- Publication
FEBS Letters, 2008, Vol 582, Issue 23/24, p3263
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.08.030