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- Title
Chimeras of bacterial translation factors Tet(O) and EF-G
- Authors
Thakor, Nehal S.; Nechifor, Roxana; Scott, Paul G.; Keelan, Monika; Taylor, Diane E.; Wilson, Kevin S.
- Abstract
Abstract: Ribosomal protection proteins (RPPs) confer bacterial resistance to tetracycline by releasing this antibiotic from ribosomes stalled in protein synthesis. RPPs share structural similarity to elongation factor G (EF-G), which promotes ribosomal translocation during normal protein synthesis. We constructed and functionally characterized chimeric proteins of Campylobacter jejuni Tet(O), the best characterized RPP, and Escherichia coli EF-G. A distinctly conserved loop sequence at the tip of domain 4 is required for both factor-specific functions. Domains 3–5: (i) are necessary, but not sufficient, for functional specificity; and (ii) modulate GTP hydrolysis by EF-G, while minimally affecting Tet(O), under substrate turnover conditions.
- Subjects
MICROBIAL proteins; ESCHERICHIA coli; CAMPYLOBACTER jejuni; PROTEINS
- Publication
FEBS Letters, 2008, Vol 582, Issue 9, p1386
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.03.023