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- Title
Identification of gonadal soma-derived factor involvement in Monopterus albus (protogynous rice field eel) sex change.
- Authors
Zhu, Yefei; Wang, Chunlei; Chen, Xiaowu; Guan, Guijun
- Abstract
We studied molecular events and potential mechanisms underlying the process of female-to-male sex transformation in the rice field eel ( Monopterus albus), a protogynous hermaphrodite fish in which the gonad is initially a female ovary and transforms into male testes. We cloned and identified a novel gonadal soma derived factor (GSDF), which encodes a member of the transforming growth factor-beta superfamily. gsdf expression was measured in gonads of female, intersex and male with reverse transcription-PCR and gsdf's role in sex transformation was studied with qPCR, histological analysis and dual-color in situ hybridization assays and compared to other sex-related genes. gsdf was correlated to Sertoli cell differentiation, indicating involvement in testicular differentiation and sex transformation from female to male in this species. A unique expression pattern reveals a potential role of gsdf essential for the sex transformation of rice field eels.
- Subjects
MONOPTERUS albus; FISH embryology; SEX differentiation (Embryology); TRANSFORMING growth factors-beta; REVERSE transcriptase polymerase chain reaction; SERTOLI cells
- Publication
Molecular Biology Reports, 2016, Vol 43, Issue 7, p629
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-016-3997-8