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- Title
Cell Cycle Dependence of Elicitor-induced Signal Transduction in Tobacco BY-2 Cells.
- Authors
Kadota, Yasuhiro; Watanabe, Takashi; Fujii, Shinsuke; Maeda, Yutaka; Ohno, Ryoko; Higashi, Katsumi; Sano, Toshio; Muto, Shoshi; Hasezawa, Seiichiro; Kuchitsu, Kazuyuki
- Abstract
The molecular links between the cell cycle and defense responses in plants are largely unknown. Using synchronized tobacco BY-2 cells, we analyzed the cell cycle dependence of elicitor-induced defense responses. In synchronized cultured apoaequorin-expressing cells, the increase in cytosolic free Ca2+ induced by a proteinaceous elicitor, cryptogein, was greatly suppressed during the G2 and M phases in comparison with G1 or S phases. Treatment with cryptogein during the G1 or S phases also induced biphasic (rapid/transient and slow/prolonged) responses in activation of mitogen-activated protein kinases (MAPKs) and production of reactive oxygen species (ROS). In contrast, elicitor treatment during the G2 or M phases induced only a rapid and transient phase of MAPK activation and ROS production. Their slow and prolonged phases as well as expression of defense-related genes, cell cycle arrest and cell death were induced only after the cell cycle progressed to the G1 phase; removal of the elicitor before the start of the G1 phase inhibited these responses. These results suggest that although cryptogein recognition occurred at all phases of the cell cycle, the recognition during the S or G1 phases, but not at the G2 or M phases, induces the prolonged activation of MAPKs and the prolonged production of ROS, followed by cell cycle arrest, accumulation of defense-related gene transcripts and cell death. Elicitor signal transduction depends on the cell cycle and is regulated differently at each phase.
- Subjects
CELL cycle; TOBACCO; CELLULAR signal transduction; CELL death; REACTIVE oxygen species; MITOGEN-activated protein kinases; GENETIC transformation
- Publication
Plant & Cell Physiology, 2005, Vol 46, Issue 1, p156
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pci008