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- Title
Induction of DNA Damage in Mouse Colorectum by Administration of Colibactin-producing Escherichia coli, Isolated from a Patient With Colorectal Cancer.
- Authors
TAKUMI NARITA; YUTA TSUNEMATSU; NORIYUKI MIYOSHI; MASAMI KOMIYA; TAKAHIRO HAMOYA; GEN FUJII; YUKO YOSHIKAWA; MICHIO SATO; MASANOBU KAWANISHI; HARUHIKO SUGIMURA; YUJI IWASHITA; YUKARI TOTSUKA; MASARU TERASAKI; KENJI WATANABE; KEIJI WAKABAYASHI; MICHIHIRO MUTOH
- Abstract
Background/Aim: Among colorectal cancerassociated intestinal microbiota, colibactin-producing (clb+) bacteria are attracting attention. We aimed to clarify the interaction between clb+ Escherichia coli and normal colorectal epithelial cells in vivo and in vitro. Materials and Methods: Five-week-old female Balb/c mice were divided in an untreated group, a group treated with clb+ E. coli isolated from a Japanese patient with colorectal cancer (E. coli-50), and a group treated with non colibactin-producing E. coli (E. coli-50/ΔclbP). Mice were sacrificed at 18 weeks of treatment. Results: Treatment with clb+ E. coli increased positivity for H2A histone family member X phosphorylated at Ser-139 (γH2AX) in epithelial cells of the luminal surface of the mouse rectum but this did not occur in the E. coli- 50/ΔclbP and untreated groups. In an in vitro setting, the ratio of apoptotic cells was increased and cell counts were reduced by treatment with clb+ E. coli more than in untreated cells and normal rat colorectal epithelial cells. Conclusion: E. coli-50 induced DNA damage in the mouse rectum, possibly by direct interaction between clb+ E. coli and normal colorectal epithelial cells. Our findings imply that regulation of clb+ E. coli infection may be a useful strategy for colorectal cancer control.
- Subjects
DNA damage; COLORECTAL cancer; ESCHERICHIA coli; GUT microbiome; EPITHELIAL cells
- Publication
In Vivo, 2022, Vol 36, Issue 2, p628
- ISSN
0258-851X
- Publication type
Article
- DOI
10.21873/invivo.12746