We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
抗体封闭细胞表面GRP78抑制厄洛替尼耐药性口腔舌鳞状细胞癌细胞的生长和转移
- Authors
商家炜; 徐振华; 王月; 苏荣健; 黄克强; 王志静
- Abstract
Objective To explore the inhibitory effect of a blocking antibody specific to cell surface glucose-regulated protein 78( GRP78) on growth and metastasis of erlotinib-resistant tongue squamous cell carcinoma cells. Methods GRP78-specific antibody was used to treat an erlotinib-resistant cell line established from a tongue squamous cell carcinoma( TCA-8113-ER) . MTT and clone formation assays were used to assess the effect of the antibody blocking on the proliferation of TCA-8113-ER cells and their response to erlotinib. Cell surface expression of GRP78 in these cells was analyzed by in-cell Western assay. Apoptosis of TCA-8113-ER cells was evaluated by flow cytometry. Invasive and metastatic potential of TCA-8113-ER cells was analyzed by scratch wound healing and transwell migration assays. Expression levels and phosphorylation states of c-MET,AKT,and STAT3 were analyzed by Western blotting. Results GRP78-specific antibody inhibited the growth and migration of TCA-8113-ER cells while inducing their apoptosis and enhancing their sensitivity to erlotinib. At the molecular level,the treatment inhibited phosphorylation of c-MET,AKT,and STAT3. Conclusion Antibody-mediated blockade of cell surface GRP78 inhibits the growth,invasiveness,and metastatic potential of erlotinib-resistant tongue squamous cell carcinoma cells while increasing their sensitivity to erlotinib.
- Publication
Journal of China Medical University, 2019, Vol 48, Issue 6, p489
- ISSN
0258-4646
- Publication type
Article
- DOI
10.12007/j.issn.0258-4646.2019.06.003