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- Title
Developmental Patterns of Ceramide Glucosyltransferase (GlcT-1) Expression in the Mouse: In Situ Hybridization Using DIG-labeled RNA Probes.
- Authors
Young-Kug Choo; Ichikawa, Shinichi; Hirabayashi, Yoshio
- Abstract
Glycosphingolipids (GSLs) play significant roles in a variety of cell membrane events, including cellular interactions, signaling, and trafficking. Ceramide glucosyltransferase (glucosylceramide synthase, GlcT-1, EC 2.4.1.80) catalyzes the initial step in GSL synthesis, the transfer of glucose from UDP-glucose to ceramide. The reaction product of glucosylceramide serves as a core structure for over 400 species of GSLs. The enzyme is a key regulatory factor controlling intracellular levels of ceramide and GSLs. Appearance and differential distribution of GlcT-1 mRNA during mice postimplantation embryogenesis |embryonic (E) days; E9, E11, E13, E15| were investigated by in situ hybridization with digoxigenin-labeled RNA probes, coupled with alkaline phosphatase detection. On E9. tissues of the mesencephalon, myelecephalon, diencephalons, and telencephaton expressed GlcT-1. On E11 , it was expressed to a detectable extent in various tissues including mesencephalon, myelece-phalon, diencephalon, telencephalon, nose, lung, liver, vertebra, tail, spinal cord, and tongue. The expression patterns of E13 were similar to those of E11, except that the heart and stomach became positive. On E15, a specific signal for GlcT-1 was detected in all organs of the embryo. These results provide the first evidence that GlcT-1 is differentially expressed during postimplantation embryogenesis.
- Subjects
CERAMIDES; GLYCOSPHINGOLIPIDS; CELL membranes; GLYCOSYLTRANSFERASES; MESENCEPHALON
- Publication
Molecules & Cells (Springer Nature), 2001, Vol 11, Issue 3, p346
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)17046-4