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- Title
PTEN hypermethylation profiles of Chinese Kazakh patients with esophageal squamous cell carcinoma.
- Authors
Pan, Q. F.; Li, W. T.; Dong, H. C.; Chen, Y. Z.; Yin, L.; Liu, W.; Wang, W. W.; Liu, D.; Li, S. G.; Gu, W. Y.; Chen, J. Z.; Yang, L.; Zhang, W. J.; Li, F.
- Abstract
Aberrant DNA methylation of promoter region CpG islands may serve as an alternative mechanism to genetic defects in the inactivation of tumor suppressor genes ( TSGs) in human malignancies. The aim of this study was to examine the promoter methylation status of the PTEN TSG and its association with esophageal squamous cell carcinoma ( ESCC) carcinogenesis in a Chinese Kazakh population, which is known to have a relatively high ESCC incidence and mortality. The methylation status of the PTEN promoter region was determined in patients with ESCC ( n = 95) and healthy individuals ( n = 65) using highly sensitive Sequenom Epityper assays. The methylation level of the PTEN gene was significantly higher in patients with ESCC than in healthy controls. The median methylation level was 10.0% (interquartile range [ IQR]: 7.0-11.0%) in patients with ESCC and 6.0% in controls ( IQR: 4.0-9.0%; P = 0.001). PTEN methylation levels were higher in male patients with ESCC than in male controls, whereas a trend toward significance was observed between female patients with ESCC and female controls ( P = 0.005 and P = 0.086, respectively). The PTEN methylation level was associated with histopathological grade and lymph node metastasis in patients with ESCC ( P = 0.002 and P = 0.009, respectively). To our knowledge, this is the first report to show the presence of PTEN promoter CpG hypermethylation in ESCC and its association with tumor metastasis.
- Subjects
PTEN protein; SQUAMOUS cell carcinoma; ESOPHAGEAL cancer; DNA methylation; CPG nucleotides; TUMOR suppressor genes; CARCINOGENESIS; LYMPHATIC metastasis
- Publication
Diseases of the Esophagus, 2014, Vol 27, Issue 4, p396
- ISSN
1120-8694
- Publication type
Article
- DOI
10.1111/dote.12106