We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The micro RNA expression profile in porcine skeletal muscle is changed by constant heat stress.
- Authors
Hao, Y.; Liu, J. R.; Zhang, Y.; Yang, P. G.; Feng, Y. J.; Cui, Y. J.; Yang, C. H.; Gu, X. H.
- Abstract
Heat stress has profound effects on animal performance and muscle function, and micro RNAs (mi RNAs) play a critical role in muscle development and stress responses. To characterize the changes in mi RNAs in skeletal muscle responding to heat stress, the mi RNA expression profiles of longissimus dorsi muscles of pigs raised under constant heat stress (30 °C; n = 8) or control temperature (22 °C; n = 8) for 21 days were analyzed by Illumina deep sequencing. A total of 58 differentially expressed mi RNAs were identified with 30 down-regulated and 28 up-regulated, and 63 differentially expressed target genes were predicted by mi RNA- mRNA joint analysis. GO and KEGG analyses showed that the genes regulated by differentially expressed mi RNAs were enriched in glucose metabolism, cytoskeletal structure and function and stress response. Real-time PCR showed that the mRNA levels of PDK4, HSP90 and DES were significantly increased, whereas those of SCD and LDHA significantly decreased by heat exposure. The protein levels of CALM1, DES and HIF1 α were also significantly increased by constant heat. These results demonstrated that the change in mi RNA expression in porcine longissimus dorsi muscle underlies the changes in muscle structure and metabolism in porcine skeletal muscle affected by constant heat stress.
- Subjects
SKELETAL muscle physiology; MICRORNA; GENE expression; PHYSIOLOGICAL effects of heat; MUSCLE growth; SWINE physiology
- Publication
Animal Genetics, 2016, Vol 47, Issue 3, p365
- ISSN
0268-9146
- Publication type
Article
- DOI
10.1111/age.12419