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- Title
Differential responses to mitogen stimulation in lymphocytes from normal individuals and Lesch-Nyhan patients: influence of the bicarbonate buffer system.
- Authors
Gausset, P. H.; Vamos, E.; Delespesse, G.; Kulakowski, S.; Ducha-Teau, J.; de Bruyn, C.
- Abstract
Three patients affected with the Lesch-Nyhan syndrome were found to have normal levels of immunoglobulins, normal numbers of circulating B anil I cells and normal IgG secretion in vitro in response to polyclonal activators. However, when cultures were performed in the absence of a bicarbonate buffer system, the proliferative response lo several T cell stimulants(phytohaemagglutinin, concanavalin A and streptokinase-streptodornase) was impaired in Lesch-Nyhan cells as judged from the incorporation of labelled thymidine, uridine and leucine. This situation could be abolished by incubation in a 5% CO2 atmosphere and even reversed by supplementation of bicarbonate to the culture medium Blocking the de novo purine synthesis by Methotrexate resulted in a more pronounced inhibition of the mitogenic response in Lesch Nyhan lymphocytes than in normal cells. The differences in proliferative response between normal and Lesch-Nyhan lymphocytes with regard to culture conditions point to the critical role of the de novo pathway in lymphocyte stimulation.
- Subjects
LESCH-Nyhan syndrome; IMMUNOGLOBULINS; T cells; PHYTOHEMAGGLUTININS; LYMPHOCYTES; BLOOD proteins
- Publication
Clinical & Experimental Immunology, 1980, Vol 42, Issue 2, p294
- ISSN
0009-9104
- Publication type
Article