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- Title
Arabidopsis hot2 encodes an endochitinase-like protein that is essential for tolerance to heat, salt and drought stresses.
- Authors
YeRim Kwon; Sun-Hee Kim; Moon-Soo Jung; Mi-Sook Kim; Jee-Eun Oh; Hyun-Woo Ju; Kang-il Kim; Vierling, Elizabeth; Hojoung Lee; Suk-Whan Hong
- Abstract
The Arabidopsis hot2 mutant was originally identified based on its lack of thermotolerance, but pleiotropic abnormal phenotypes are also exhibited under normal conditions, including semi-dwarfism, ethylene overproduction and aberrant cell shape with incomplete cell walls. Here we present additional characterization of the hot2 mutant, and the map-based cloning of HOT2. Mutants of hot2 had an aberrant tolerance to salt and drought stresses, and accumulated high levels of Na+ in cells under either normal or NaCl stress conditions. Expression of the stress-inducible COR15A and KIN1 gene in hot2 mutants in response to increased NaCl concentrations was normal. HOT2 encoded a chitinase-like protein (AtCTL1) that has not previously been shown to be involved in tolerance to salt stress. Ten-day-old seedlings of wild-type plants exhibited constitutive expression of the AtCTL1 transcript, the level of which was unaffected by treatment with NaCl, mannitol or mild heat. These observations provide genetic evidence that a chitinase-like protein prevents the overaccumulation of Na+ ions, thereby contributing to the salt tolerance in Arabidopsis. A possible role for this chitinase-like protein in Arabidopsis tolerance to abiotic stress is discussed.
- Subjects
ARABIDOPSIS; DROUGHT tolerance; MOLECULAR cloning; GENETIC engineering; CHITINASE; GROWTH disorders
- Publication
Plant Journal, 2007, Vol 49, Issue 2, p184
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2006.02950.x