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- Title
Distinct post-transcriptional modifications result into seven alternative transcripts of the CC-NBS-LRR geneJA1trofPhaseolus vulgaris.
- Authors
Ferrier-Cana, Elodie; Macadré, Catherine; Sévignac, Mireille; David, Perrine; Langin, Thierry; Geffroy, Valérie
- Abstract
The generation of splice variants has been reported for various plant resistance (R) genes, suggesting that these variants play an important role in disease resistance. Most of the time these R genes belong to the Toll and mammalian IL-1 receptor-nucleotide-binding site-leucine-rich repeat (TIR-NBS-LRR) class of R genes. InPhaseolus vulgaris, a resistance gene cluster (referred to as theB4R-gene cluster) has been identified at the end of linkage group B4. At this complex resistance cluster, three R specificities (Co-9,Co-yandCo-z) and two R QTLs effective against the fungal pathogenColletotrichum lindemuthianum, the causal agent of anthracnose, have been identified. At the molecular level, four resistance gene candidates encoding putative full-length, coiled-coil (CC)-NBS-LRR R-like proteins, with LRR numbers ranging from 18 to 20, have been previously characterized. In the present study, seven cDNA corresponding to truncated R-like transcripts, belonging to the CC-NBS-LRR class of plant disease R genes, have been identified. These seven transcripts correspond to a single gene namedJA1tr, which encodes, at most, only five LRRs. The sevenJA1trtranscript variants result from distinct post-transcriptional modifications ofJA1tr, corresponding to alternative splicing events of two introns, exon skipping and multiple ‘aberrant splicing’ events in the open reading frame (ORF).JA1trwas mapped at theB4R-gene cluster identified in common bean. These post-transcriptional modifications of the single geneJA1trcould constitute an efficient source of diversity. The present results provide one of the few reports of transcript variants with truncated ORFs resulting from a CC-NBS-LRR gene.
- Subjects
COMMON bean; KIDNEY bean; POST-translational modification; GENETIC translation; PROTEIN synthesis; GENETICS; PLANT genetics
- Publication
Theoretical & Applied Genetics, 2005, Vol 110, Issue 5, p895
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-004-1908-1