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- Title
Quantification of trans-zeatin in corn wastes and liquid organic fertilizers by HPLC chromatography.
- Authors
Chóez, I.; Manzano Santana, P.; Peralta, E.
- Abstract
The trans-zeatin and other phytohormones are used as growth regulators to be induced in plants. Their presence has been determined in corn grains and some plant species, but not in their waste or liquid organic fertilizers, so the objective of this investigation was to quantify the compound by high performance liquid chromatography (HPLC). Ethanol extracts of corn waste and bioinsumos were prepared. Were identified by thin layer chromatography on aluminum chromatoplates Merck 60 F254 10×10cm as stationary phase and methanolacetonitrile (80:20) as mobile phase. Their quantification was performed by HPLC on a C18 column, 250mm ×4 mm (5 μm), and methanolwater (40:60) acidified 5% acetic acid as mobile phase, 0.8 mL.min-1 flow rate and wavelength of 280 ηm. The extract of corn grains was taken as control. Statistical analysis to validate the results obtained was performed in the InfoStac software. The control sample was found to have the highest concentration of trans-zeatin (0.730 mg/g). These results have not been referenced in the literature and allow adding ecological and economic utility of this type of agricultural waste.
- Subjects
ZEATIN; ORGANIC fertilizers; HIGH performance liquid chromatography; AGRICULTURAL wastes; THIN layer chromatography
- Publication
Emirates Journal of Food & Agriculture (EJFA), 2014, Vol 26, Issue 9, p813
- ISSN
2079-052X
- Publication type
Article
- DOI
10.9755/ejfa.v26i9.18455