We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sensitive HIV-1 DNA Pol Next-Generation Sequencing for the Characterisation of Archived Antiretroviral Drug Resistance.
- Authors
Botha, Johannes C.; Byott, Matthew; Spyer, Moira J.; Grant, Paul R.; Gärtner, Kathleen; Chen, Wilson X.; Burton, James; Bamford, Alasdair; Waters, Laura J.; Giaquinto, Carlo; Turkova, Anna; Vavro, Cindy L.; Nastouli, Eleni
- Abstract
Modern HIV-1 treatment effectively suppresses viral amplification in people living with HIV. However, the persistence of HIV-1 DNA as proviruses integrated into the human genome remains the main barrier to achieving a cure. Next-generation sequencing (NGS) offers increased sensitivity for characterising archived drug resistance mutations (DRMs) in HIV-1 DNA for improved treatment options. In this study, we present an ultra-sensitive targeted PCR assay coupled with NGS and a robust pipeline to characterise HIV-1 DNA DRMs from buffy coat samples. Our evaluation supports the use of this assay for Pan-HIV-1 analyses with reliable detection of DRMs across the HIV-1 Pol region. We propose this assay as a new valuable tool for monitoring archived HIV-1 drug resistance in virologically suppressed individuals, especially in clinical trials investigating novel therapeutic approaches.
- Subjects
NUCLEOTIDE sequencing; DRUG resistance; ANTIRETROVIRAL agents; HIV; HIV-positive persons
- Publication
Viruses (1999-4915), 2023, Vol 15, Issue 9, p1811
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v15091811