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- Title
CITOLOGIA DE LAVADO BRONCOALVEOLAR DE CÃES: COMPARAÇÃO ENTRE LÂMINAS A FRESCO E CONSERVADAS EM FORMOL.
- Authors
Ribas, Cristina Rauen; Dornbusch, Peterson Triches; Cirio, Silvana Maris; Mangrich Rocha, Rita Maria Venâncio; Achcar Capriglione, Luiz Guilherme; D'Amico Fam, Ana Laura Pinto
- Abstract
The bronchoalveolar lavage in dogs is a diagnostic method recommended in cases of illnesses of the caudal respiratory tract, when routine exams do not allow a diagnosis. The exam relies on the cytological analysis and fresh smear immediately after sampling, which may difficult the adequate processing at the specialized laboratories. Accordingly, a sample conservation media which increases the material time span to be analyzed would be desirable. In the present study, bronchoalveolar lavage was performed in fourteen healthy adult dogs. Samples were separate in two aliquots: one freshly made by in chamber of sedimentation, and another processed one week later, with the sample preserved in formalin. Slides were stained by quick Romanovsky-type stain (Panótico Rápido®, Laborclin®, Pinhais, Brazil). The infused volume, the recovered volume, the macroscopic aspect, the counting of nucleated cells, the differential cell analysis and the smear descriptive analysis such as cellularity, mucus presence, erythrocytes, leukocytes and degenerate cells were evaluated. The statistical analysis was performed with T test, for paired samples with p<0.05. Significant increase was found in the amount of lymphocytes and decrease of the macrophage numbers in formalin preserved samples. All other observations were similar in both groups. Therefore, the preservation of the bronchoalveolar lavage sample of dogs in formalin, during one week, preserved the cells, making the bronchoalveolar lavage technique routinely feasible.
- Subjects
BRONCHOALVEOLAR lavage; DOGS; FORMALDEHYDE; CYTOLOGY; MUCUS; MACROPHAGES
- Publication
Archives of Veterinary Science, 2010, Vol 15, Issue 2, p69
- ISSN
1517-784X
- Publication type
Article
- DOI
10.5380/avs.v15i2.14880