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- Title
Mitochondrial density contributes to the immune response of macrophages to lipopolysaccharide via the MAPK pathway
- Authors
Kasahara, Emiko; Sekiyama, Atsuo; Hori, Mika; Hara, Kenjiro; Takahashi, Nozomi; Konishi, Masami; Sato, Eisuke F; Matsumoto, Sohkichi; Okamura, Haruki; Inoue, Masayasu
- Abstract
Abstract: We investigated the role of mitochondrial reactive oxygen species (ROS) in the response of macrophages to lipopolysaccharide (LPS) using RAW 264.7 cells and their ρo cells lacking mitochondria. Mitochondrial density, respiratory activity and related proteins in ρo cells were significantly lower than those in RAW cells. LPS rapidly stimulated mitochondrial ROS prior to cytokine secretion, such as TNF-α and IL-6, from RAW 264.7 cells by activating the MAPK pathway, while the response was attenuated in ρo cells. Exposure of ρo cells to H2O2 partially restored the secretion of cytokines induced by LPS. These results suggest that mitochondrial density and/or the respiratory state contribute to intracellular oxidative stress, which is responsible for the stimulation of LPS-induced MAPK signaling to enhance cytokine release from macrophages.
- Subjects
MITOCHONDRIA; IMMUNE response; MACROPHAGES; ENDOTOXINS; MITOGEN-activated protein kinases; CELL receptors; TUMOR necrosis factors; NF-kappa B
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2263
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.049