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- Title
LRRK2 directly phosphorylates Akt1 as a possible physiological substrate: Impairment of the kinase activity by Parkinson’s disease-associated mutations
- Authors
Ohta, Etsuro; Kawakami, Fumitaka; Kubo, Makoto; Obata, Fumiya
- Abstract
Abstract: LRRK2 is the causal molecule for autosomal-dominant familial Parkinson’s disease, although its true function, including its physiological substrates, remains unknown. Here, using in vitro kinase assay with recombinant proteins, we demonstrated for the first time that LRRK2 directly phosphorylates Akt1, a central molecule involved in signal transduction for cell survival and prevention of apoptosis. Ser473, one of two amino acids essential for Akt1 activation, was the target site for LRRK2. A knockdown experiment using intact cells also demonstrated LRRK2-mediated phosphorylation of Akt1 (Ser473), suggesting that Akt1 is a convincing candidate for the physiological substrate of LRRK2. The disease-associated mutations, R1441C, G2019S, and I2020T, exhibited reduced interaction with, and phosphorylation of, Akt1, suggesting one possible mechanism for the neurodegeneration caused by LRRK2 mutations. Structured summary of protein interactions: LRRK2 phosphorylates Akt1 by protein kinase assay (View Interaction 1, 2, 3). LRRK2 phosphorylates MBP by protein kinase assay (View Interaction 1, 2). LRRK2 binds to Akt1 by pull down (View Interaction 1, 2, 3).
- Subjects
PARKINSON'S disease; GENETIC mutation; LEUCINE; PROTEIN kinases; PHOSPHORYLATION; CELLULAR signal transduction; APOPTOSIS prevention; AMINO acids
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2165
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.044