We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Recombinant plant gamma carbonic anhydrase homotrimers bind inorganic carbon
- Authors
Martin, Victoria; Villarreal, Fernando; Miras, Isabelle; Navaza, Alda; Haouz, Ahmed; González-Lebrero, Rodolfo M.; Kaufman, Sergio B.; Zabaleta, Eduardo
- Abstract
Abstract: Gamma carbonic anhydrases (γCA) are widespread in Prokaryotes. In Eukaryotes, homologous genes were found only in plant genomes. In Arabidopsis and maize, the corresponding gene products are subunits of mitochondrial Complex I. At present, only γCA homotrimers of Methanosarcina thermophila (CAM) show reversible carbon dioxide (CO2) hydration activity. In the present work, it is shown that recombinant plant γCA2 could form homotrimers and bind . However, they are unable to catalyse the reversible hydration of CO2. These results suggest that plant γCAs do not act as carbonic anhydrases but with a related activity possibly contributing to recycle CO2 in the context of photorespiration. Structured summary: MINT-7266044: gamma CA2 (uniprotkb:Q9C6B3) and gamma CA2 (uniprotkb:Q9C6B3) physically interact (MI:0914) by dynamic light scattering (MI:0038) MINT-7266036: gamma CA2 (uniprotkb:Q9C6B3) and gamma CA2 (uniprotkb:Q9C6B3) physically interact (MI:0914) by molecular sieving (MI:0071)
- Subjects
RECOMBINANT proteins; CARBONATES; MITOCHONDRIA; ARABIDOPSIS thaliana; CARBON dioxide; CARBONIC anhydrase; PROKARYOTES; PLANT genomes; PLANT photorespiration
- Publication
FEBS Letters, 2009, Vol 583, Issue 21, p3425
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.09.055