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- Title
Laccase from Melanocarpus albomyces binds effectively to cellulose
- Authors
Kiiskinen, Laura-Leena; Palonen, Hetti; Linder, Markus; Viikari, Liisa; Kruus, Kristiina
- Abstract
A laccase from the thermophilic fungus Melanocarpus albomyces was shown to bind to softwood and pure microcrystalline cellulose. The binding isotherm fitted well the Langmuir type one-site binding model. The adsorption parameters indicated that M. albomyces laccase binds with high affinity to cellulose with a relatively low maximum binding capacity, as compared to the values for various cellulases. The binding was shown to be reversible and not influenced by non-specific protein or 0.1–0.5 M Na2SO4. No binding was detected with laccases from Trametes hirsuta or Mauginiella sp., which suggests that binding to cellulose is typical for only some laccases.
- Subjects
WOOD chemistry; LACCASE; OXIDOREDUCTASES; CELLULOSE
- Publication
FEBS Letters, 2004, Vol 576, Issue 1/2, p251
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.08.040