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- Title
Clinicopathological significance of indoleamine 2,3-dioxygenase 1 expression in colorectal cancer.
- Authors
Ferdinande L; Decaestecker C; Verset L; Mathieu A; Moles Lopez X; Negulescu AM; Van Maerken T; Salmon I; Cuvelier CA; Demetter P; Ferdinande, L; Decaestecker, C; Verset, L; Mathieu, A; Moles Lopez, X; Negulescu, A-M; Van Maerken, T; Salmon, I; Cuvelier, C A; Demetter, P
- Abstract
<bold>Background: </bold>Indoleamine 2,3-dioxygenase 1 (IDO1) is a tryptophan-catabolising enzyme that induces immune tolerance by modulating T-cell responses. Carcinomas may create an immunosuppressive state via IDO1 expression. Here we examined a possible contribution of IDO1 on this phenomenon and investigated whether IDO1 has prognostic value in colorectal cancer (CRC).<bold>Methods: </bold>IDO1 expression was investigated by quantitative PCR and western blotting in three colon cancer cell lines, in basal state and after interferon (IFN)-γ stimulation. Semi-quantitative immunohistochemistry was used to evaluate IDO1 expression in 265 pT1-4N0-2Mx-staged CRCs. Results were related to clinical variables and correlated with amounts of CD3(+) and CD8(+) T lymphocytes, which were quantitatively evaluated using image analysis.<bold>Results: </bold>In vitro expression of IDO1 depended on IFN-γ stimulation. Higher IDO1 expression at the tumour invasion front was an independent adverse prognostic factor in pT1-4N1Mx-staged CRC. It was associated with overall survival (P=0.001) and with metachronous metastases (P=0.018). IDO1 expression was not associated with the presence of CD3(+) or CD8(+) T lymphocytes.<bold>Conclusion: </bold>Higher IDO1 expression at the tumour invasion front is involved in CRC progression and correlates with impaired clinical outcome, suggesting that IDO1 is an independent prognostic indicator for CRC.
- Publication
British Journal of Cancer, 2012, Vol 106, Issue 1, p141
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/bjc.2011.513