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- Title
Clearance of Chlamydia pneumoniae Infection in H-2 Class I<sup>–/–</sup> Human Leucocyte Antigen-A2.1 Monochain Transgenic Mice.
- Authors
Tammiruusu, A.; Haveri, A.; Pascolo, S.; Lahesmaa, R.; Stevanovic, S.; Rammensee, H.-G.; Sarvas, M.; Puolakkainen, M.; Vuola, J. M.
- Abstract
CD8+ T cells have been suggested to play an important role in protective immunity against pulmonary Chlamydia pneumoniae infection in mice. Moreover, several classical major histocompatibility complex class I – restricted cytotoxic CD8+ T lymphocytes (CTL) specific for C. pneumoniae– derived peptides have been identified. Here, we studied the outcome of C. pneumoniae infection in human leucocyte antigen (HLA)-A2.1 transgenic mice (HHD mice) that are only able to express a classical human class I molecule (HLA-A2.1). C. pneumoniae infection was self-restricted in HHD mice which were able to develop specific immune responses and a protective immunity against a subsequent rechallenge in a manner comparable to wildtype mice. Furthermore, accumulation of functional and C. pneumoniae-specific T cells to the site of infection was detected after challenge. Antigen processing and HLA-A2.1-dependent presentation was studied by immunizing the HHD mice with chlamydial outer protein N (CopN). Isolation of a peptide-specific CTL line from the CopN-immunized mice suggests that the HLA-A2.1 molecule can support the development of CTL response against a chlamydial protein in mice. These findings suggest that the transgenic mouse model can be used for further characterization of the HLA-A2.1-restricted CD8+ T-cell response during C. pneumoniae infection and for identification of CD8 epitopes from chlamydial antigens.
- Subjects
CHLAMYDOPHILA pneumoniae infections; LABORATORY mice; LYMPHOCYTES; LEUCOCYTES; IMMUNOLOGY
- Publication
Scandinavian Journal of Immunology, 2005, Vol 62, Issue 2, p131
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2005.01645.x