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- Title
Increased accumulation of CD30 ligand-positive mast cells associates with eosinophilic inflammation in nasal polyps.
- Authors
Zhai, Guan‐Ting; Li, Jing‐Xian; Zhang, Xin‐Hao; Liao, Bo; Lu, Xiang; Liu, Zheng; Zhai, Guan-Ting; Li, Jing-Xian; Zhang, Xin-Hao
- Abstract
<bold>Objective: </bold>Activation of mast cells associates with eosinophilic inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP). The disease-specific mast cell-triggering mechanisms apart from immunoglobulin E are poorly understood in CRSwNP. CD30L/CD30 are members of the tumor necrosis factor/receptor superfamily and display immune modulatory function on mast cells. The aim of this study was to explore the expression and function of CD30 and CD30L in CRSwNP.<bold>Methods: </bold>The mRNA expression of CD30 and CD30L was analyzed by real-time polymerase chain reaction. The cellular expression of CD30L was determined by immunofluorescence staining. The soluble CD30 levels in nasal tissues were detected by enzyme-linked immunosorbent assay. HMC-1 cells, a human mast cell line, were cultured and stimulated with CD30.<bold>Results: </bold>Compared with control tissues, CD30 mRNA expression levels were increased in eosinophilic polyps, and soluble CD30 protein levels were upregulated in both eosinophilic and noneosinophilic polyps with a greater increase in eosinophilic type. CD30 was expressed by T cells and B cells in nasal polyps. The CD30L mRNA expression levels and the number of CD30L+ cells and CD30L+ tryptase+ mast cells were increased in eosinophilic polyps but not in noneosinophilic polyps as compared with control tissues. Mast cells accounted for 60% of CD30L+ cells in eosinophilic polyps. CD30 induced HMC-1 cells to produce interleukin (IL)-4 and IL-13 without degranulation. Mast cells expressed IL-4 and IL-13 in eosinophilic polyps. The number of CD30L+ tryptase+ mast cells was positively correlated with the number of eosinophils and total inflammatory cells in eosinophilic polyps.<bold>Conclusion: </bold>CD30/CD30L-mediated mast cell activation may promote the eosinophilic inflammation in CRSwNP.<bold>Level Of Evidence: </bold>NA Laryngoscope, 129:E110-E117, 2019.
- Subjects
MAST cells; EOSINOPHILIA; NASAL polyps; TUMOR necrosis factors; ENZYME-linked immunosorbent assay; IMMUNOGLOBULIN E; BIOCHEMISTRY; CELL culture; CELLULAR signal transduction; CHRONIC diseases; COMPARATIVE studies; CYTOKINES; FLUORESCENT antibody technique; IMMUNOGLOBULINS; PHENOMENOLOGY; RESEARCH methodology; MEDICAL cooperation; POLYMERASE chain reaction; RESEARCH; RHINITIS; RNA; SINUSITIS; EVALUATION research; CASE-control method
- Publication
Laryngoscope, 2019, Vol 129, Issue 3, pE110
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.27658