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- Title
Cytotoxicity, Cell Cycle Arrest, Antioxidant Activity and Interaction of Dibenzoxanthenes Derivatives with DNA.
- Authors
Wang, Xiu-Zhen; Yang, Bao-Yun; Lin, Gan-Jian; Xie, Yang-Yin; Huang, Hong-Liang; Liu, Yun-Jun
- Abstract
In this study, we report the DNA interaction and cytotoxicity of four dibenzoxanthene compounds 1-4. The binding behaviors of these compounds to calf thymus DNA were studied by absorption titration, viscosity measurements. The DNA binding constants of compounds 1, 2, 3, and 4 are 5.05×104, 2.13×103, 5.10×104, and 3.03×103 M−1, respectively. The lipophilicity of the compounds was determined by the shake flask method. The cytotoxicity of these compounds has been assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. These compounds exhibit high activity against BEL-7402, Hela, MG-63, and SKBR-3 cells. The cell cycle arrest was analyzed by flow cytometry. These compounds inhibit S phase of BEL-7402 and SKBR-3 cells. The experiments on antioxidant activity show that these compounds exhibit good antioxidant activity against hydroxyl radical (•OH). Four compounds of this family bind DNA, are cytotoxic in an in vitro assay of mitochondrial potential, show antioxidant activity, and cause damage during S phase in cell lines.
- Subjects
CELL-mediated cytotoxicity; CELL cycle; XANTHENE; DNA-binding proteins; ANTIOXIDANTS; TETRAZOLIUM compounds; ABSORPTION
- Publication
DNA & Cell Biology, 2012, Vol 31, Issue 9, p1468
- ISSN
1044-5498
- Publication type
Article
- DOI
10.1089/dna.2012.1726