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- Title
Wort disinfection treatment with electron beam for bioethanol production.
- Authors
Perez Calegari, Rubens; Alberto da Silva, Eric; Maria da Silva, Ana Paula; Pego Gomes, Marcelo; Amorim Mota, Layna; Arthur, Valter; Sampaio Baptista, Antonio
- Abstract
Microbial contamination of the wort during the fermentation process causes significant losses in ethanol production worldwide and creates a dependence of the industry on chemicals and antibiotics to control contamination. Therefore, this study used electron beam (e-beam) to disinfect wort from sugarcane (Saccharum officinarum L.) molasses and investigate the bioethanol fermentation. Four treatments (T0 - T3) were carried out using ionizing doses of radiation through the electron accelerator: 0 (control), 10, 20, and 40 kGy. Total mesophiles, total bacteria, sucrose, glucose, fructose, phenolics, flavonoids, hydroxymethylfurfural (5-HMF), and Furfural were measured. An alcoholic fermentation assay was performed after the irradiation process. The irradiated treatments showed no inversion of sugars and formation of the inhibitory by-products flavonoids, furfural and 5-HMF, except for the phenolic compounds. The lower dose tested (10 kGy) reduced more than 99.9 % of the total mesophiles and more than 99.99 % of the total bacteria in the substrate. In the fermentation, the irradiated worts presented similar (p > 0.05) yields (92, 93, and 94 %) and ethanol productivity levels (0.89, 0.88, and 0.87 g L-1 h-1, for T1, T2, and T3 respectively). However, all treatments presented higher yields and productivity (p < 0.05) when compared to the control (88 % and 0.85 g L-1 h-1), highlighting the possible use of e-beam in wort fermentation at a lower dose (10 kGy). This allows reduction in losses caused by microbial contamination, besides increasing fermentation yield and productivity with lower energy consumption.
- Subjects
ETHANOL as fuel; MICROBIAL contamination; SUGARCANE; FERMENTATION; ELECTRON accelerators; ELECTRON beams; FURFURAL; ETHANOL
- Publication
Scientia Agricola, 2023, Vol 80, p1
- ISSN
0103-9016
- Publication type
Article
- DOI
10.1590/1678-992X-2021-0260