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- Title
The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment.
- Authors
Sztatelman, Olga; Grzyb, Joanna; Gabryś, Halina; Banaś, Agnieszka Katarzyna
- Abstract
Background: Ultraviolet B (UV-B) irradiation can influence many cellular processes. Irradiation with high UV-B doses causes chlorophyll degradation, a decrease in the expression of genes associated with photosynthesis and its subsequent inhibition. On the other hand, sublethal doses of UV-B are used in post-harvest technology to prevent yellowing in storage. To address this inconsistency the effect of short, high-dose UV-B irradiation on detached Arabidopsis thaliana leaves was examined. Results: Two different experimental models were used. After short treatment with a high dose of UV-B the Arabidopsis leaves were either put into darkness or exposed to constant light for up to 4 days. UV-B inhibited dark-induced chlorophyll degradation in Arabidopsis leaves in a dose-dependent manner. The expression of photosynthesis-related genes, chlorophyll content and photosynthetic efficiency were higher in UV-B -treated leaves left in darkness. UV-B treatment followed by constant light caused leaf yellowing and induced the expression of senescence-related genes. Irrespective of light treatment a high UV-B dose led to clearly visible cell death 3 days after irradiation. Conclusions: High doses of UV-B have opposing effects on leaves depending on their light status after UV treatment. In darkened leaves short UV-B treatment delays the appearance of senescence symptoms. When followed by light treatment, the same doses of UV-B result in chlorophyll degradation. This restricts the potential usability of UV treatment in postharvest technology to crops which are stored in darkness.
- Subjects
EFFECT of ultraviolet radiation on plants; ARABIDOPSIS; LEAF physiology; CHLOROPHYLL; PHOTOSYNTHETIC rates; CELLULAR aging
- Publication
BMC Plant Biology, 2015, Vol 15, p1
- ISSN
1471-2229
- Publication type
Article
- DOI
10.1186/s12870-015-0667-2