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- Title
Regulation of carbon metabolic fluxes to enhance lipid and succinate production in oleaginous fungus Mortierella alpina.
- Authors
Sun, Chongran; Yang, Tao; Zhang, Shuangfei; Wen, Qikun; Gao, Binyuan; Liu, Qianzi; Cheng, Haina; Wang, Yuguang; Chen, Zhu; Zhou, Hongbo
- Abstract
Mortierella alpina is popular for lipid production, but the low carbon conversion rate and lipid yield are major obstacles for its economic performance. Here, external addition of organic acids involved in tricarboxylic acid cycle was used to tune carbon flux and improve lipid production. Citrate was determined to be the best organic acid that can be used for enhancing lipid production. By the addition of citrate, the lipid titer and content were approximately 1.24 and 1.34 times higher, respectively. Meanwhile, citrate supplement also promoted the accumulation of succinate, an important value-added platform chemical. Owing to the improved lipid and succinate production through adding citrate, the carbon conversion rate of M. alpina reached up to 52.17%, much higher than that of the control group (14.11%). The addition of citrate could redistribute carbon flux by regulating the expression level of genes related to tricarboxylic acid cycle metabolism. More carbon fluxes flow to lipid and succinate synthesis, which greatly improved the carbon conversion efficiency of M. alpina. This study provides an effective and straightforward strategy with potential economic benefits to improve carbon conversion efficiency in M. alpina.
- Publication
World Journal of Microbiology & Biotechnology, 2024, Vol 40, Issue 10, p1
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-024-04082-z