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- Title
Inhibition of SARS-CoV-2 infection and reduction of cell death by polyene.
- Authors
Alcaraz Estrada, Sofia Lizeth; Blake, Iván Ortega; Hernández, Arturo Galván; Cobos, Jorge Hernández; Estrada Toledo, Nancy Viridiana; Alvarado Flores, Raúl Ubaldo; García Hernández, Montserrat Elemí; Sarmiento Silva, Rosa Elena
- Abstract
As infections from emerging or reemerging virus became a serious threat to humans in the last years, drug repurposing, is an a promising, fast and cost-effective alternative that can overcome traditional de novo drug discovery and development challenges. Given that therapeutic options for antiviral treatment of SARS-CoV-2 remain limited, through this strategy we sought to find molecules with antiviral activity. In this regard, antifungals such as itraconazole, anidulafungin, and micafungin were reported to have antiviral activity against SARS-CoV-2. Polyene antibiotics are potent antifungal agents currently used in human therapy. Among these is Amphotericin B (AmB), and its newly created less derivative A21. In addition, lipid-based formulations have been developed to deliver polyenes, such as liposomes and these alternatives are also available for AmB and A21. Therefore, it was decided to see if these compounds have antiviral activity against SARS-CoV-2 through a time-of-drug-addition strategy. We were able to observe an 83 % reduction of viral particles determine by plaque assay, when AmB is added after infection at a concentration of 10 µM. No significant reduction of the viral particles was detected with the other compounds, however, a reduction in cell death due to SARS-CoV-2 infection was observed, but only when viruses are treated before infection. The latter could be explained if other signaling pathways related to viral entry and cell death are being stimulated. Exploring these alternative pathways could provide valuable information for the development of future antiviral molecules.
- Publication
Veterinaria México OA, 2024, Vol 11, p94
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305