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- Title
Isolation And Characterization Of Anti-Sars-Cov-2 Omicron Antibodies From A Semi-Immune Phage Display Library.
- Authors
Mendoza-Salazar, I; Gómez-Castellano, K. M.; González-González, E.; Gamboa-Suasnavart R., R.; Rodríguez-Luna, S. D.; Santiago-Casas, G.; Cortés-Paniagua, M. I.; Pérez-Tapia, S. M.; Almagro, J. C.
- Abstract
We describe the discovery and characterization of antibodies with potential broad SARS-CoV-2 neutralization profiles. The antibodies were obtained from a phage display library built with the VH repertoire of a convalescent COVID-19 patient who was infected with SARS-CoV-2 B.1.617.2 (Delta). The patient received a single dose of Ad5-nCoV vaccine (ConvideciaTM, CanSino Biologics Inc.) one month before developing COVID-19 symptoms. Four synthetic VL libraries were used as counterparts of the immune VH repertoire. After three rounds of panning with SARS-CoV-2 receptor-binding domain wildtype (RBD-WT) 34 unique scFvs, were identified, with 27 cross-reactive for the RBD-WT and RBD Delta (RBD-DT), and seven specifics for the RBD-WT. The cross-reactive scFvs were more diverse than the RBD-WT specific ones, being encoded by several IGHV genes from the IGHV1 and IGHV3 families combined with short HCDR3s. Three cross-reactive scFvs and one RBD-WT specific scFv were converted to human IgG1 (hIgG1). The four antibodies blocked the RBD-WT binding to angiotensin converting enzyme 2 (ACE2). Importantly, one of the antibodies also recognized the RBD from the B.1.1.529 (Omicron) isolate, implying that the VH repertoire of the convalescent patient would protect against SARS-CoV-2 Wildtype, Delta, and Omicron. From a practical viewpoint, the triple cross-reactive antibody provides the substrate for developing therapeutic antibodies with a broad SARS-CoV-2 neutralization profile.
- Subjects
CORONAVIRUS diseases; IMMUNOGLOBULINS; CORONAVIRUSES
- Publication
Veterinaria México OA, 2024, Vol 11, p31
- ISSN
2448-6760
- Publication type
Abstract
- DOI
10.22201/fmvz.24486760e.2024.1304