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- Title
SCM-198 ameliorates the quality of postovulatory and maternally aged oocytes by reducing oxidative stress.
- Authors
Ma, Wei; Zhao, Xi; Wang, Qingxin; Wu, Xue; Yang, Tingting; Chen, Yuqi; Zhu, Yizhun; Wang, Xia
- Abstract
Oocyte aging is a key constraint on oocyte quality, leading to fertilization failure and abnormal embryonic development. In addition, it is likely to generate unfavorable assisted reproductive technology (ART) outcomes. SCM-198, a synthetic form of leonurine, was found to rescue the rate of oocyte fragmentation caused by postovulatory aging. Therefore, the aim of this study was to conduct a more in-depth investigation of SCM-198 by exploring its relationship with aged oocytes after ovulation or maternal aging and clarifying whether it affects cell quality. The results indicate that, compared to the postovulatory aged group, the 50 µM SCM-198 group significantly improved sperm-egg binding and increased fertilization of aged oocytes, restoring the spindle apparatus/chromosome structure, cortical granule distribution, and ovastacin and Juno protein distribution. The 50 µM SCM-198 group showed significantly normal mitochondrial distribution, low levels of reactive oxygen species (ROS), and a small quantity of early oocyte apoptosis compared to the postovulatory aged group. Above all, in vivo supplementation with SCM-198 effectively eliminated excess ROS and reduced the spindle/chromosome structural defects in aged mouse oocytes. In summary, these findings indicate that SCM-198 inhibits excessive oxidative stress in oocytes and alters oocyte quality both in vitro and in vivo.
- Subjects
EMBRYOLOGY; SPINDLE apparatus; CHROMOSOME structure; REACTIVE oxygen species; REPRODUCTIVE technology
- Publication
Journal of Ovarian Research, 2024, Vol 17, Issue 1, p1
- ISSN
1757-2215
- Publication type
Article
- DOI
10.1186/s13048-024-01504-2