A bstract: Many pathological conditions involve alterations in expression of nicotinic acetylcholine receptors (nAChRs). The following studies were designed to investigate cellular mechanisms involved with expression and turnover of α3β4* nAChRs. These studies support constitutive turnover of adrenal α3β4* nAChRs and the use of cultured adrenal chromaffin cells to study nAChR regulation.