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- Title
Efficient enzymatic production of rebaudioside A from stevioside.
- Authors
Wang, Yu; Chen, Liangliang; Li, Yan; Li, Yangyang; Yan, Ming; Chen, Kequan; Hao, Ning; Xu, Lin
- Abstract
Stevioside and rebaudioside A are the chief diterpene glycosides present in the leaves ofStevia rebaudiana. Rebaudioside A imparts a desirable sweet taste, while stevioside produces a residual bitter aftertaste. Enzymatic synthesis of rebaudioside A from stevioside can increase the ratio of rebaudioside A to stevioside in steviol glycoside products, providing a conceivable strategy to improve the organoleptic properties of steviol glycoside products. Here, we demonstrate the efficient conversion of stevioside to rebaudioside A by coupling the activities of recombinant UDP-glucosyltransferase UGT76G1 fromS. rebaudianaand sucrose synthase AtSUS1 fromArabidopsis thaliana. The conversion occurred via regeneration of UDP-glucose by AtSUS1. UDP was applicable as the initial material instead of UDP-glucose for UDP-glucose recycling. The amount of UDP could be greatly reduced in the reaction mixture. Rebaudioside A yield in 30 h with 2.4 mM stevioside, 7.2 mM sucrose, and 0.006 mM UDP was 78%. Concept of the two-enzyme system for efficient synthesis of rebaudioside A from stevioside. UGT76G1, UDP-glucosyltransferase fromStevia rebaudiana; AtSUS1, sucrose synthase fromArabidopsis thaliana.
- Subjects
STEVIOSIDE; ENZYMES; CHEMICAL synthesis; SUCROSE synthase
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 1, p67
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2015.1072457